Molecular Imaging of Mesothelioma with T 99m c -ECG and G 68 a -ECG

• Published in: BioMed research international Vol. 2012; pp. 1 - 9
• Main Authors: Zhang, Yin-Han, Bryant, Jerry, Kong, Fan-Lin, Yu, Dong-Fang, Mendez, Richard, Edmund Kim, E., Yang, David J.
• Format: Journal Article
• Language: English
• Published: New York Hindawi Limited 01.01.2012
• Subjects: Asbestos; Binding sites; Biomedical research; Cancer therapies; Medical imaging; NMR; Nuclear magnetic resonance; Oncology; Proteins; Studies; Tomography
• ISSN: 1110-7243; 2314-6133; 1110-7251; 2314-6141
• DOI: 10.1155/2012/232863

We have developed ethylenedicysteine-glucosamine (ECG) as an alternative to 18 F-fluoro-2-deoxy-D-glucose ( 18 F-FDG) for cancer imaging. ECG localizes in the nuclear components of cells via the hexosamine biosynthetic pathway. This study was to evaluate the feasibility of imaging mesothelioma with T 99m c -ECG and G 68 a -ECG. ECG was synthesized from thiazolidine-4-carboxylic acid and 1,3,4,6-tetra-O-acetyl-2-amino-D-glucopyranose, followed by reduction in sodium and liquid ammonia to yield ECG (52%). ECG was chelated with T 99m c /tin (II) and G 68 a / 69 Ga chloride for in vitro and in vivo studies in mesothelioma. The highest tumor uptake of T 99m c -ECG is 0.47 at 30 min post injection, and declined to 0.08 at 240 min post injection. Tumor uptake (%ID/g), tumor/lung, tumor/blood, and tumor/muscle count density ratios for T 99m c -ECG (30–240 min) were 0.47 ± 0.06 to 0.08 ± 0.01 ; 0.71 ± 0.07 to 0.85 ± 0.04 ; 0.47 ± 0.03 to 0.51 ± 0.01 , and 3.49 ± 0.24 to 5.06 ± 0.25 ; for G 68 a -ECG (15–60 min) were 0.70 ± 0.06 to 0.92 ± 0.08 ; 0.64 ± 0.05 to 1.15 ± 0.08 ; 0.42 ± 0.03 to 0.67 ± 0.07 , and 3.84 ± 0.52 to 7.00 ± 1.42 ; for 18 F-FDG (30–180 min) were 1.86 ± 0.22 to 1.38 ± 0.35 ; 3.18 ± 0.44 to 2.92 ± 0.34 , 4.19 ± 0.44 to 19.41 ± 2.05 and 5.75 ± 2.55 to 3.33 ± 0.65 , respectively. Tumor could be clearly visualized with T 99m c -ECG and G 68 a -ECG in mesothelioma-bearing rats. T 99m c -ECG and G 68 a -ECG showed increased uptake in mesothelioma, suggesting they may be useful in diagnosing mesothelioma and also monitoring therapeutic response.