Comparison of gene expression in Solanum bulbocastanum infected with virulent and avirulent isolates of Meloidogyne chitwoodi

Resistance to root knot nematode M. chitwoodi has been identified in the wild tuber-bearing Solanum species, S. bulbocastanum. Three pathotypes were identified suggesting at least two different genetic factors for virulence and resistance in the pathogen and the host species, respectively. Roots of...

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Published inPlant protection science Vol. 38; no. SI 2 - 6th Conf EFPP; pp. 721 - 722
Main Authors Wishart, J., Phillips, M.S., Paterson, A., Blok, V.C.
Format Journal Article
LanguageEnglish
Published Czech Academy of Agricultural Sciences 31.12.2002
Subjects
meloidogyne chitwoodi
solanum bulbocastanum
suppression subtractive hybridisation
virulence genes
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Summary:Resistance to root knot nematode M. chitwoodi has been identified in the wild tuber-bearing Solanum species, S. bulbocastanum. Three pathotypes were identified suggesting at least two different genetic factors for virulence and resistance in the pathogen and the host species, respectively. Roots of S. bulbocastanum were infested with two isolates of M. chitwoodi differing in virulence. The infection process was monitored by histological examination of roots allowing time points to be identified. cDNA libraries were constructed from infected root tissue using Suppression Subtractive Hybridisation (SSH) to enrich for transcripts from either compatible or incompatible interactions, at three days and seven days post infection. Both plant and nematode genes, which may be important during the host/parasite interaction, were identified.
ISSN:1212-2580
1805-9341
DOI:10.17221/10599-PPS