Expression of ASC splice variant found in Japanese patients with palindromic rheumatism is regulated by rs8056505 single nucleotide polymorphism and interleukin-1 beta

• Published in: Asian Pacific journal of allergy and immunology
• Main Authors: Hattori, Masaya, Yabuuchi, Atsuko, Tanaka, Hayate, Kawara, Taketo, Wang, Hongyan, Inoue, Koji, Shiozawa, Shunichi, Komai, Koichiro
• Format: Journal Article
• Language: English
• Published: Thailand 22.08.2022
• ISSN: 0125-877X; 2228-8694
• DOI: 10.12932/AP-010322-1339

Palindromic rheumatism (PR) is an infrequent form of periodic arthritis. Based on the similarity of the pathogenesis of PR to autoinflammatory syndromes, we previously found that the dominant-active splice variant of the inflammasome adaptor protein, apoptosis-associated speck-like protein containing a CARD (ASC), which lacks exon 2 (Δexon2), is expressed in Japanese patients with PR. Elucidation of the mechanism of Δexon2 ASC production and the effect of IL-1β on splicing. The genomic DNA of Japanese patients with PR was sequenced. The effect of the observed single nucleotide polymorphisms (SNPs) on ASC splicing was determined via exon trapping using THP-1 cells stimulated with interleukin-1 beta (IL-1β) or ceramide. To investigate the genes that affect alternative splicing via IL-1β, we analyzed the transcriptome of IL-1β-treated THP-1 cells using RNA sequencing. We found the rs8056505 A->G SNP located in the 5'-untranslated region of the genomic ASC gene in patients and that Δexon2 expression was induced by this SNP, whereas it was suppressed by IL-1β or ceramide. We detected 131,426 transcripts and identified 52 differentially expressed genes (DEGs) consisting of 41 downregulated genes and 11 upregulated genes in IL-1β-stimulated THP-1 cells. The splicing-related gene MASCRNA was the most significantly induced gene by IL-1β. We propose a cyclic expression model in which ASC alternates between wild-type and Δexon2 expression regulated by the rs8056505 G allele and splicing factors induced by IL-1β. This cycle may be correlated with the formation of periodic PR pathologies.