A Rapid Diagnosis for Periodontitis Based on Enzymatic Activity of Periodontopathic Bacteria Clinical specificity and cut-off value of SK-013

• Published in: Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) Vol. 33; no. 1; pp. 154 - 163
• Main Authors: OHTAKE, Tohru, TAKANO, Tokiko, KURIHARA, Chikako, MINAMIZAKI, Nobuki, MIYASHITA, Hajime, HASEGAWA, Kohji, SUGITA, Noriko, SATOH, Etsuko, TAI, Hideaki, KOBAYASHI, Tetsuo, YOSHIE, Hiromasa, HARA, Kohji, ISODA, Ryuutaro, KAWAI, Toshihisa, SAHO, Teruyuki, OGO, Shuuji, MIKI, Yasuo, OKADA, Hiroshi
• Format: Journal Article
• Language: Japanese
• Published: JAPANESE SOCIETY OF PERIODONTOLOGY 28.03.1991
• Subjects: Clinical specificity; Cut-off value; Macroscopic test; Periodontopathic bacteria; SK-013
• ISSN: 0385-0110; 1880-408X
• DOI: 10.2329/perio.33.154

Various laboratory techniques have been applied to the microbiological diagnosis of periodontal disease. However, certain disadvantages persist. In this study we examined the specific peptidase activity in periodontal pockets by using the chair-side test system, SK-013 as a microbiological diagnostics technique. SK-013 can rapidly (within 15 min) evaluate the peptidase activities derived from periodontopathic bacteria such as Treponema denticola and Bacteroides species. We applied SK-013 to patients with periodontitis, gingivitis, dental caries, other types of periodontal infectious disease and periodontally healthy controls and then evaluated the clinical specificity of SK-013, assessing its cut-off level. SK-013 activity was determined both spectrophotometrically and by using the color standard. Phase contrast microscopy was also utilized for determination of subgingival microorganisms. SK -013 activity was specifically detected in the periodontitis group. Setting its cut-off level at 0.2 Try U/ml based on the number of spirochetes in the subgingival pocket, we got good sensitivity, specificity, predictive value and efficacy in terms of the diagnostic value of SK-013. Using the same cut -off level, a good coincidence was also shown in both spectrophotometrically and by using the color standard. These results indicate that SK-013 is a useful chair-side diagnostic method for periodontal disease.