Effect of abomasal butyrate infusion on gene expression in the duodenum of lambs

A previous study infusing butyrate into the abomasum of sheep produced increased oxygen, glucose, glutamate, and glutamine uptake by the portal-drained viscera. These changes were thought to be partially due to increases in glycolysis and cell proliferation. The purpose of this study was to evaluate...

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Bibliographic Details
Published inJournal of animal science Vol. 95; no. 3; p. 1191
Main Authors Foote, A. P., Zarek, C. M., Kuehn, L.A., Cunningham, H. C., Cammack, K. M., Freetly, H. C., Lindholm-Perry, A. K.
Format Journal Article
LanguageEnglish
Published Champaign Oxford University Press 01.03.2017
Subjects
Abomasum
Animal sciences
Biosynthesis
Carnitine
Cell proliferation
D-Galactosamine
Digestive system
DNA microarrays
Duodenum
Fatty acids
Feeding
Gene clusters
Gene expression
Genes
Glucose
Glutamine
Glycolysis
Metabolism
Mitochondria
Mucosa
Oxygen
Oxygen uptake
Ribonucleic acid
RNA
Saline solutions
Sheep
Viscera
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Summary:A previous study infusing butyrate into the abomasum of sheep produced increased oxygen, glucose, glutamate, and glutamine uptake by the portal-drained viscera. These changes were thought to be partially due to increases in glycolysis and cell proliferation. The purpose of this study was to evaluate the duodenum transcriptome of control and butyratetreated lambs to determine whether genes involved in these pathways were altered. Polled Dorset lambs (n = 9) received a pulse dose of either butyrate (10 mg/ kg BW) or an equal volume of a buffered saline solution (1 mL/kg BW) daily at the time of feeding. Lambs were euthanized approximately 4 h after treatment/ feeding on d 21, and a sample of duodenal mucosa was obtained from which total RNA was isolated for microarray analysis. A total of 230 genes were differentially expressed (P < 0.05). Pathway analyses performed with the differentially expressed genes revealed glycolysis, fatty acid activation/biosynthesis, UDP-N-acetyl-D- galactosamine biosynthesis, γ-Linolenate biosynthesis, and mitochondrial L-carnitine shuttle pathways up-regulated by the butyrate treatment. Additionally, expression of functional gene clusters related to mitochondrial function was found to be enriched (P < 0.05) with the butyrate treatment. These data could partially explain the metabolite flux changes that were observed with the butyrate treatment; specifically the increase in glucose uptake and glycolysis pathway upregulation and the increased oxygen uptake and upregulation of mitochondria function-related genes.
ISSN:1525-3163
0021-8812
1525-3163
DOI:10.2527/jas2016.1022