Multiple regulatory genes in the salinomycin biosynthetic gene cluster ofStreptomyces albus CCM 4719

A DNA fragment containing part of the salinomycin biosynthetic gene cluster from industrial strainStreptomyces albus CCM 4719 was cloned. Sequence analysis of the 25.809-kbp fragment revealed the presence of 8 open reading frames (ORFs), including two large ORFs encoding three modular sets of oligok...

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Bibliographic Details
Published inFolia microbiologica Vol. 52; no. 4; pp. 359 - 365
Main Authors Knirschová, R., Nováková, R., Fecková, L., Timko, J., Turňa, J., Bistáková, J., Kormanec, J.
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.07.2007
Subjects
Bacteriology
Candicidin
Clusters
DNA
Genes
Nystatin
Open reading frames
Salinomycin
Sequence analysis
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Summary:A DNA fragment containing part of the salinomycin biosynthetic gene cluster from industrial strainStreptomyces albus CCM 4719 was cloned. Sequence analysis of the 25.809-kbp fragment revealed the presence of 8 open reading frames (ORFs), including two large ORFs encoding three modular sets of oligoketide synthase, followed by three genes (salRI, salRII, salRIII) encoding transcriptional regulators. The first two regulators, SalRI and SalRII, belonged to the novel LAL family of large transcriptional regulators. SalRIII was highly similar to the NysRIV, AmphRIV, and FscRI transcriptional regulators from the oligoene macrolides nystatin, amphotericin, and R008/candicidin clusters, respectively.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:0015-5632
1874-9356
DOI:10.1007/BF02932090