Glucosylglycerate is an osmotic solute and an extracellular metabolite produced byStreptomyces caelestis

Streptomyces caelestis DSM 40084produces two osmolytes,viz. 2-O-(α-d-glucopyranosyl)-ζ-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in...

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Published inFolia microbiologica Vol. 52; no. 5; pp. 451 - 456
Main Authors Pospíšil, S., Halada, P., Petříček, M., Sedmera, P.
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.09.2007
Subjects
Ammonium sulfate
Bacteria
Glycerol
Intracellular levels
Magnetic resonance spectroscopy
Mass spectrometry
Mass spectroscopy
Metabolites
NMR
NMR spectroscopy
Nuclear magnetic resonance
Osmolarity
Sodium chloride
Streptomyces
Trehalose
Yeasts
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Summary:Streptomyces caelestis DSM 40084produces two osmolytes,viz. 2-O-(α-d-glucopyranosyl)-ζ-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2× in response to a step change of medium osmolarity caused by 0.3 % NaCl.1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is aS. caelestis metabolite commonly released from the cells; its concentration reached 3 g/L during the cultivation in a yeast extract-(NH4)2SO4-glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genusStreptomyces and on its free excretion to the medium.
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ISSN:0015-5632
1874-9356
DOI:10.1007/BF02932103