Removal of beta-lactam antibiotics during growth of Escherichia coli strains hosting pUR and pEX vectors
Beta-lactam antibiotics supplied at 200 mu g/mL were rapidly removed from culture fluids of E. coli strains hosting pUR and pEX vectors, indicating that a substantial period of growth occurred in the absence of positive selection. The retention period of the drugs depended on the specific activity a...
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Published in | Biotechnology techniques Vol. 1; no. 3; pp. 157 - 162 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
01.09.1987
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Subjects | |
Online Access | Get full text |
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Summary: | Beta-lactam antibiotics supplied at 200 mu g/mL were rapidly removed from culture fluids of E. coli strains hosting pUR and pEX vectors, indicating that a substantial period of growth occurred in the absence of positive selection. The retention period of the drugs depended on the specific activity and type of the beta-lactamase, the nature of the antibiotic and growth rate of the host. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0951-208X 1573-6784 |
DOI: | 10.1007/BF00227553 |