Removal of beta-lactam antibiotics during growth of Escherichia coli strains hosting pUR and pEX vectors

Beta-lactam antibiotics supplied at 200 mu g/mL were rapidly removed from culture fluids of E. coli strains hosting pUR and pEX vectors, indicating that a substantial period of growth occurred in the absence of positive selection. The retention period of the drugs depended on the specific activity a...

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Bibliographic Details
Published inBiotechnology techniques Vol. 1; no. 3; pp. 157 - 162
Main Authors Kemp, G.W., Britz, M.L.
Format Journal Article
LanguageEnglish
Published 01.09.1987
Subjects
beta -lactam antibiotics
cloning vectors
Escherichia coli
plasmids
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Summary:Beta-lactam antibiotics supplied at 200 mu g/mL were rapidly removed from culture fluids of E. coli strains hosting pUR and pEX vectors, indicating that a substantial period of growth occurred in the absence of positive selection. The retention period of the drugs depended on the specific activity and type of the beta-lactamase, the nature of the antibiotic and growth rate of the host.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00227553