P18 Glutaminolysis and Steatosis to Steatohepatitis Transition: Role of Glutaminase

Gls gene expression, Gls activity and protein expression were assessed by qPCR, fluorometric and IHC assays. The length of a microsatellite in the promoter region of GLS was assessed by capillary electrophoresis (long allele>14 GCA repeats). Results: Hepatic Gls expression was found to increase a...

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Published inThe American journal of gastroenterology Vol. 118; no. 9S; p. S13
Main Authors García-Fernández, Vanessa, Gómez, Antonio Gil, Rojas, Ángela, Muñoz-Hernández, Rocío, Gato, Sheila, Montero-Vallejo, Rocío, Gallego-Durán, Rocío, Maya-Miles, Douglas, Rico, María del Carmen, Ampuero, Javier, Romero-Gómez, Manuel
Format Journal Article
LanguageEnglish
Published New York Wolters Kluwer Health Medical Research, Lippincott Williams & Wilkins 01.09.2023
Subjects
Ammonia
Biopsy
Gastroenterology
Liver cirrhosis
Multivariate analysis
Protein expression
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Summary:Gls gene expression, Gls activity and protein expression were assessed by qPCR, fluorometric and IHC assays. The length of a microsatellite in the promoter region of GLS was assessed by capillary electrophoresis (long allele>14 GCA repeats). Results: Hepatic Gls expression was found to increase along with the progression of NAFLD (NASH fold-4.04±1.33; P=0.019 and P=0.033, respectively), being associated with ballooning (P=0.006) and significant fibrosis (P=0.019).
ISSN:0002-9270
1572-0241
DOI:10.14309/01.ajg.0000948356.42903.bf