Zinc uptake in swine intestinal brush border membrane vesicles using a 65Zn/69mZn duel isotope experiment

Supplemental zinc as ZnO is routinely added to nursery pig diets at 15 to 20 times the nutritional requirement to alleviate physiological stress and enhance performance. The mechanism by which Zn accomplishes this function is unknown, however, high concentrations of Zn are excreted in the environmen...

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Bibliographic Details
Published inJournal of radioanalytical and nuclear chemistry Vol. 276; no. 1; pp. 129 - 134
Main Authors Huntington, C. E., Veum, T. L., Morris, J. S.
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Netherlands 01.04.2008
Subjects
Chemistry
Chemistry and Materials Science
Diagnostic Radiology
Hadrons
Heavy Ions
Inorganic Chemistry
Nuclear Chemistry
Nuclear Physics
Physical Chemistry
Brush Border Membrane Vesicle
Zinc Uptake
Competitive Uptake
Liquid Scintillation Spectrometer
Intestinal Brush Border Membrane Vesicle
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Summary:Supplemental zinc as ZnO is routinely added to nursery pig diets at 15 to 20 times the nutritional requirement to alleviate physiological stress and enhance performance. The mechanism by which Zn accomplishes this function is unknown, however, high concentrations of Zn are excreted in the environment at undesirable levels. To study Zn uptake, we developed a multi-stage digestion model followed by exposure to swine intestinal brush border membrane vesicles (BBMV). We report on the feasibility of using a duel label ( 65 Zn and 69m Zn) to simultaneously quantify the competitive uptake of Zn from co-existing zinc supplements using our BBMV model.
ISSN:0236-5731
1588-2780
DOI:10.1007/s10967-007-0421-9