Hydrogen-producing conditions and mutation mechanisms of a highly efficient mutant strain Ethanoligenens harbinense YR-3

In this study, the optimal hydrogen (H2) production conditions of the high-efficiency H2-producing mutant strain Ethanoligenens harbinense YR-3 (carbon-nitrogen ratio 5.5, phosphate buffer 80 mM, initial pH 6.0, biotin 1.4 mg/L) are obtained by intermittent experiments. The maximum specific H2 produ...

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Bibliographic Details
Published inJournal of bioscience and bioengineering
Main Authors Zheng, Guoxiang, Tao, Dongxu, Ren, Nanqi
Format Journal Article
LanguageEnglish
Published Elsevier B.V 22.08.2024
Subjects
Conditions optimization
Hydrogen production mechanism
Proteomics
Hydrogen production mechanism
Conditions optimization
Proteomics
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Summary:In this study, the optimal hydrogen (H2) production conditions of the high-efficiency H2-producing mutant strain Ethanoligenens harbinense YR-3 (carbon-nitrogen ratio 5.5, phosphate buffer 80 mM, initial pH 6.0, biotin 1.4 mg/L) are obtained by intermittent experiments. The maximum specific H2 production rate of YR-3 (2.85 mol H2/mol glucose) was 1.4 times that of the wild strain ZGX4 (2.04 mol H2/mol glucose). The liquid-phase products are mainly ethanol and acetic acid, indicating that the metabolic pathway has not changed. Two-dimensional electrophoresis and mass spectrometry were used to compare and analyze the protein map differences between YR-3 and ZGX4. The results show that 1,6-fructose diphosphate aldolase and the flavoprotein in hydrogenase are highly expressed. This study will provide a theoretical basis for the genetic modification of high-efficiency H2-producing strains and the improvement of H2 production capacity.
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content type line 23
ISSN:1389-1723
1347-4421
1347-4421
DOI:10.1016/j.jbiosc.2024.05.012