The Mouseformin(Fmn) Gene: Genomic Structure, Novel Exons, and Genetic Mapping

• Published in: Genomics (San Diego, Calif.) Vol. 39; no. 3; pp. 303 - 311
• Main Authors: Wang, Cindy C., Chan, David C., Leder, Philip
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.02.1997
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1996.4519

Mutations in the mouseformin(Fmn) gene, formerly known as thelimb deformity(ld) gene, give rise to recessively inherited limb deformities and renal malformations or aplasia. TheFmngene encodes many differentially processed transcripts that are expressed in both adult and embryonic tissues. To study the genomic organization of theFmnlocus, we have usedFmnprobes to isolate and characterize genomic clones spanning 500 kb. Our analysis of these clones shows that theFmngene is composed of at least 24 exons and spans 400 kb. We have identified two novel exons that are expressed in the developing embryonic limb bud as well as adult tissues such as brain and kidney. We have also used a microsatellite polymorphism from within theFmngene to map it genetically to a 2.2-cM interval between D2Mit58 and D2Mit103.