Cofilin Activation during Ca2+-Triggered Secretion from Adrenal Chromaffin Cells

Cofilin is one of the major actin depolymerizing proteins in eukaryotic cells and involved in many membrane modulating activities, such as cell growth and motility. Here we examined whether cofilin is activated upon Ca2+ regulated noradrenalin secretion from bovine adrenal chromaffin cells. We found...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 286; no. 3; pp. 493 - 498
Main Authors Birkenfeld, Jörg, Kartmann, Bettina, Betz, Heinrich, Roth, Dagmar
Format Journal Article
LanguageEnglish
Published Elsevier Inc 24.08.2001
Subjects
actin
catecholamine secretion
Lim kinase
actin
Lim kinase
catecholamine secretion
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Summary:Cofilin is one of the major actin depolymerizing proteins in eukaryotic cells and involved in many membrane modulating activities, such as cell growth and motility. Here we examined whether cofilin is activated upon Ca2+ regulated noradrenalin secretion from bovine adrenal chromaffin cells. We found that triggering exocytosis by nicotine causes a dephosphorylation and thereby activation of cofilin. Furthermore, in permeabilized chromaffin cells the addition of Ca2+ alone is sufficient to trigger both, regulated exocytosis and cofilin activation. This is consistent with cofilin activation being required for actin reorganization during exocytosis.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.5435