Heme Synthesizing Enzymes ofPlasmodium knowlesi:A Simian Malarial Parasite

Intraerythrocytic stages of cell-freePlasmodium knowlesipossess significant activities of heme biosynthetic enzymes, viz. δ-aminolevulinic acid synthase (δ-ALAS), δ-aminolevulinic acid dehydrase (δ-ALAD), ferrochelatase (FC), and tryptophan pyrrolase (enzyme representing free heme pool). δ-Aminolevu...

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Bibliographic Details
Published inExperimental parasitology Vol. 88; no. 1; pp. 60 - 63
Main Authors Srivastava, Pratima, Pandey, V.C.
Format Journal Article
LanguageEnglish
Published Elsevier Inc 01.01.1998
Subjects
Plasmodium knowlesi
δ-ALAS, δ-aminolevulinic acid synthase
ACD, acid citrate dextrose
TP, tryptophan pyrrolase
FC, ferrochelatase
antimalarials
Heme synthesis
hemin
δ-ALAD, δ-aminolevulinic acid dehydrase
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Summary:Intraerythrocytic stages of cell-freePlasmodium knowlesipossess significant activities of heme biosynthetic enzymes, viz. δ-aminolevulinic acid synthase (δ-ALAS), δ-aminolevulinic acid dehydrase (δ-ALAD), ferrochelatase (FC), and tryptophan pyrrolase (enzyme representing free heme pool). δ-Aminolevulinic acid synthase and FC showed higher activities in schizont than in ring trophozoite stages. Uninfected monkey erythrocytes did not possess the above-mentioned enzyme activities; on the contrary, leucocytes showed detectable enzyme activities. δ-Aminolevulinic acid synthase was not appreciably inhibited by different antimalarials. Succinyl acetone and hemin exhibited a concentration-dependent inhibition of δ-ALAD and δ-ALAS, respectively.
ISSN:0014-4894
1090-2449
DOI:10.1006/expr.1998.4193