Excitotoxic Brain Lesion Modifies Binding to a USF Binding Site Acting as a Negative Regulatory Element in theProtease Nexin-1Promoter
The expression of the serine protease inhibitorProtease nexin-1 (PN-1)is upregulated in glial cells following different types of lesion in the nervous system. A strong negative regulatory element has been shown by the missing nucleoside technique to be a CACGTG site (E-box) in the proximal part of t...
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Published in | Molecular and cellular neuroscience Vol. 8; no. 1; pp. 28 - 37 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Inc
01.07.1996
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Online Access | Get full text |
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Summary: | The expression of the serine protease inhibitorProtease nexin-1 (PN-1)is upregulated in glial cells following different types of lesion in the nervous system. A strong negative regulatory element has been shown by the missing nucleoside technique to be a CACGTG site (E-box) in the proximal part of thePN-1promoter. The factor binding to this site is specifically recognized by antibodies directed against the human upstream stimulatory factor (USF). Point mutations in the E-box binding site which abolish USF bindingin vitroincrease the transcriptional activity of thePN-1promoter. Cotransfection of aPN-1promoter/reporter construct together with an expression vector for humanUSF1confirms the negative regulatory function of this site. Finally, we show that the binding to this USF site changed after ibotenic acid-induced lesion of the caudate putamen in the rat brain. |
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ISSN: | 1044-7431 1095-9327 |
DOI: | 10.1006/mcne.1996.0041 |