Structure of Catechol 2,3-Dioxygenase Gene Encoded in TOM Plasmid ofPseudomonas cepaciaG4

The catechol 2,3-dioxygenase is an extradiol-type dioxygenase which cleaves the C-C bond at themetaposition of catechol to form 2-hydroxymuconic semialdehyde. A catechol 2,3-dioxygenase gene (tomB) in the TOM plasmid ofP. cepaciaG4 has been cloned and its nucleotide sequence was analyzed. The enzyme...

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Published inBiochemical and biophysical research communications Vol. 234; no. 3; pp. 578 - 581
Main Authors Oh, Jeong Mi, Kang, Eunja, Min, Kyung Rak, Kim, Chi-Kyung, Kim, Young-Chang, Lim, Jai-Yun, Lee, Ki-Sung, Min, Kyung-Hee, Kim, Youngsoo
Format Journal Article
LanguageEnglish
Published Elsevier Inc 29.05.1997
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Summary:The catechol 2,3-dioxygenase is an extradiol-type dioxygenase which cleaves the C-C bond at themetaposition of catechol to form 2-hydroxymuconic semialdehyde. A catechol 2,3-dioxygenase gene (tomB) in the TOM plasmid ofP. cepaciaG4 has been cloned and its nucleotide sequence was analyzed. The enzyme gene consisted of 945 base pairs with an ATG initiation codon and a TGA termination codon which can encode a polypeptide of molecular weight 35 kDa containing 314 amino acid residues, and a putative ribosome-binding sequence was identified at approximately 10 nucleotides upstream of the initiation codon. The deduced amino acid sequence of catechol 2,3-dioxygenase fromP. cepaciaG4 exhibited 79-82% homologies with those of 3-methylcatechol 2,3-dioxygenase ofP. putidaUCC2 and catechol 2,3-dioxygenase ofP. pickettiiPKO1.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1997.6680