RETRACTED ARTICLE: ß2-adrenoreceptor Inverse Agonist Down-regulates Muscarine Cholinergic Subtype-3 Receptor and Its Downstream Signal Pathways in Airway Smooth Muscle Cells in vitro

Mechanisms underlying ß 2 -adrenoreceptor (ß 2 AR) inverse agonist mediated bronchoprotectiveness remain unknown. We incubated ICI118,551, formoterol, budesonide, and formoterol plus budesonide, as well as ICI118,551 or pindolol plus formoterol, ICI118,551 plus forskolin, SQ22,536 or H89 plus formot...

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Published inScientific reports Vol. 7; no. 1; pp. 1 - 11
Main Authors Luo, Jian, Liu, Yuan-hua, Luo, Wei, Luo, Zhu, Liu, Chun-tao
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 04.01.2017
Nature Publishing Group
Subjects
13/51
692/420/256/2515
692/699/1785/31
82/1
82/29
82/80
Adrenergic receptors
Budesonide
Corticoids
Corticosteroids
Formoterol
Forskolin
Humanities and Social Sciences
Inverse agonists
multidisciplinary
Muscarine
Pindolol
Science
Smooth muscle
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Summary:Mechanisms underlying ß 2 -adrenoreceptor (ß 2 AR) inverse agonist mediated bronchoprotectiveness remain unknown. We incubated ICI118,551, formoterol, budesonide, and formoterol plus budesonide, as well as ICI118,551 or pindolol plus formoterol, ICI118,551 plus forskolin, SQ22,536 or H89 plus formoterol in ASMCs to detect expressions of M 3 R, PLCß 1 and IP 3 . The level of M 3 R in the presence of 10 -5 ?mmol/L ICI118,551 were significantly decreased at 12?h, 24?h and 48?h (P? ?0.05), but SQ22,536 significantly antagonized the formoterol-induced M 3 R expression (P?<?0.05). In conclusions, ß 2 AR inverse agonist, ICI118,551, exerts similar bronchoprotective effects to corticosteroids via decreasing the expression of M 3 R and inhibiting the production of IP 3 .
ISSN:2045-2322
2045-2322
DOI:10.1038/srep39905