Application of polymerase chain reaction with disposable amplicon detection device for identification of regulatory gene introduced into genetically modified maize

• Published in: Applied biological chemistry Vol. 54; no. 6; pp. 860 - 864
• Main Authors: Woo, Hee-Jong, Chung, Chan-Mi, Shin, Kong-Sik, Lim, Myung-Ho, Lee, Ki-Jong, Cho, Yong-Gu, Kweon, Soon-Jong, Suh, Seok-Cheol
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.12.2011; Springer Nature B.V; 한국응용생명화학회
• Subjects: Amplification; Applied Microbiology; Biochemistry; Biological Techniques; Bioorganic Chemistry; Cauliflowers; Chemistry; Chemistry and Materials Science; Conserved sequence; Corn; Deoxyribonucleic acid; DNA; Genetic modification; Genetically engineered organisms; Genetically modified organisms; Immunoassays; Instrumentation; Nucleotide sequence; Polymerase chain reaction; Viruses; 농학; duplex polymerase chain reaction; detection; regulatory gene; genetically modified
• ISSN: 1738-2203; 2468-0834; 2234-344X; 2468-0842
• DOI: 10.1007/BF03253173

A novel genetically modified organism (GMO) detection method was developed using a simple and rapid procedure to identify a regulatory gene introduced into genetically modified (GM) maize. After DNA extraction from GM maize flours, fragments of the conserved sequence of the cauliflower masaic virus (CaMV) 35S promoter gene along with a competitive internal control gene were amplified using duplex polymerase chain reaction (PCR). Amplification products were then detected using a disposable detection device. The quantitative detection limit for GM maize 59122 was found to be 1.0% (w/w). Because the combination-method involving PCR technology coupled with a disposable detection device does not require expensive instrumentation or expertise, it will serve as a valuable alternative to immunoassays and traditional PCR-based tests in the detection of GMOs.