Acclimation and Characterization of a Pseudomonas Strain for Improved Phosphorus Removal
A Pseudomonas strain（named as P.PAO-1） with phosphorous removal function was isolated and characterized.A new method of two-stage cultivation was applied to the strain to induce the synthesis of intracellular polyhydroxylbutyrate（PHB） in the cells.In the first stage,bacterial cells were enriched und...
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| Published in | 东华大学学报（英文版） Vol. 33; no. 3; pp. 403 - 410 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China
30.06.2016
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| Subjects | |
| Online Access | Get full text |
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| Summary: | A Pseudomonas strain（named as P.PAO-1） with phosphorous removal function was isolated and characterized.A new method of two-stage cultivation was applied to the strain to induce the synthesis of intracellular polyhydroxylbutyrate（PHB） in the cells.In the first stage,bacterial cells were enriched under a high carbon source condition;in the second stage,the bacteria cells were cultivated under the nutrient-imbalanced conditions with the carbon source-regulated medium.As a result,the PHB content of strain P.PAO-1 reached 22.8% compared with the normal 7.41% in the non-acclimated strain.This change had led to the rising of P uptake from 1 to 25 mg·L~（-1）.When added the strain P.PAO-1into the activated sludge（with the addition proportion of 1：1）,the ratio of biological phosphorus removal increased by 14.9%under the normal alternating anaerobic/aerobic conditions with low-carbon consumption.The results demonstrated that the isolated pure culture strain P.PAO-1 belonged to the functional group of poly-P accumulating microorganism.When cultured by the two-stage cultivation method,the initial accumulation of PHB in the strain cells could be achieved and the phosphorous removal capacity of strain P.PAO-1 could be induced subsequently.When applied to the wastewater,strain P.PAO-1 performed phosphorus removal from the wastewater with or without the addition of activated sludge. |
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| Bibliography: | 31-1920/N phosphorus accumulating organism（PAO） carbon sourceregulated medium enrichment ami acclimation Pseudomonas Putida QIN Hua-xing, TIAN Qing, LI Fang, ZHU Yan-bin, YANG Bo（ College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China） A Pseudomonas strain（named as P.PAO-1） with phosphorous removal function was isolated and characterized.A new method of two-stage cultivation was applied to the strain to induce the synthesis of intracellular polyhydroxylbutyrate（PHB） in the cells.In the first stage,bacterial cells were enriched under a high carbon source condition;in the second stage,the bacteria cells were cultivated under the nutrient-imbalanced conditions with the carbon source-regulated medium.As a result,the PHB content of strain P.PAO-1 reached 22.8% compared with the normal 7.41% in the non-acclimated strain.This change had led to the rising of P uptake from 1 to 25 mg·L~（-1）.When added the strain P.PAO-1into the activated sludge（with the addition proportion of 1：1）,the ratio of biological phosphorus removal increased by 14.9%under the normal alternating anaerobic/aerobic conditions with low-carbon consumption.The results demonstrated that the isolated pure culture strain P.PAO-1 belonged to the functional group of poly-P accumulating microorganism.When cultured by the two-stage cultivation method,the initial accumulation of PHB in the strain cells could be achieved and the phosphorous removal capacity of strain P.PAO-1 could be induced subsequently.When applied to the wastewater,strain P.PAO-1 performed phosphorus removal from the wastewater with or without the addition of activated sludge. |
| ISSN: | 1672-5220 |