In situ Crosslinked Hydrogel as Drug Carrier for Promoting Angiogenesis

• Published in: 东华大学学报（英文版） Vol. 34; no. 1; pp. 118 - 121
• Main Author: 张骞骞 陈良 周小军 秦明 苗莹珂 何创龙
• Format: Journal Article
• Language: English
• Published: College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,China 28.02.2017
• Subjects: sphingosine 1-phosphate (S1P); drug delivery; hydrogel; angiogenesis
• ISSN: 1672-5220

Hydrogels composed of natural polysaccharides hold great potential as a release system for delivery of small molecule drugs. In this study, the functional drug sphingosine 1-phosphate （SIP） was loaded in the hydrogel which was derived from aldehyde hyaluroulc acid （AHA） and carboxymethyl chitosun （CC） through Schiff＇s base reaction for promoting ungiogenesis. The scanning electron microscopy （SEM） images demonstrated the suitable threedimension network structure of hydrogel. The rheological properties and compressive stress-strain behavior of hydrngel were also examined, and the results indicated that the hydrogei possessed good mechanical properties. In vitro drug release behaviors showed that drug released from the hydrogei in a sustained manner and could achieve prolonged release time compared to the pure drug. The 3- （ 4, 5-dimethylthiazol-2-yl ） -2, 5-diphenyl （MTT） assay showed the good cytocompatibility of the resulting hydrogel. More importantly, the chicken chorioallantoic membrane （CAM） assay confirmed that the SIP loaded hydrogel could significantly promote angingenesis in the CAM model. As a result, our results strongly suggested that the as-prepared hydrogel would be a good candidate for delivery of functional drugs.