Sub-lethal insecticide exposure affects host biting efficiency of Kdr-resistant Anopheles gambiae

The massive use of insecticide-treated nets (ITNs) has drastically changed the environment for malaria vector mosquitoes, challenging their host-seeking behaviour and biting success. Here, we investigated the effect of a brief exposure to an ITN on the biting behaviour of Anopheles gambiae mosquitoe...

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Bibliographic Details
Published inbioRxiv
Main Authors Diop, Malal Mamadou, Chandre, Fabrice, Rossignol, Marie, Porciani, Angelique, Chateau, Mathieu, Moiroux, Nicolas, Pennetier, Cedric
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 01.07.2020
Subjects
Anopheles gambiae
Biting behavior
Culicidae
Deltamethrin
Disease transmission
Feeding
Genotypes
Host searching behavior
Insecticides
Malaria
Mosquitoes
Mutation
Nets
Permethrin
Pyrethroids
Success
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Summary:The massive use of insecticide-treated nets (ITNs) has drastically changed the environment for malaria vector mosquitoes, challenging their host-seeking behaviour and biting success. Here, we investigated the effect of a brief exposure to an ITN on the biting behaviour of Anopheles gambiae mosquitoes and the interaction between such behaviour and the kdr mutation that confers resistance to pyrethroids. To this aim, we developed a video assay to study the biting behaviour of mosquitoes with similar genetic background, but different kdr locus genotypes (SS i.e. homozygous susceptible, RS i.e. heterozygous and RR i.e. homozygous resistant), after a brief exposure to either control untreated nets or one of two types of pyrethroid-treated nets (deltamethrin or permethrin). In presence of untreated nets, the kdr mutation did not influence mosquito blood feeding success but caused differences in feeding and prediuresis durations and blood meal size. Exposure to deltamethrin ITN decreased the blood feeding success rate of RR and RS mosquitoes, whereas in presence of permethrin ITN, the kdr mutation increased the blood-feeding success of mosquitoes. Exposure to the two types of pyrethroid-treated nets reduced feeding duration, prediuresis duration and blood meal size of all three genotypes. Our study demonstrates a complex interaction between insecticide exposure and the kdr mutation on the biting behavior of mosquitoes, which may substantially impact malaria vector fitness and disease transmission. Competing Interest Statement The authors have declared no competing interest. Footnotes * Version 5 of this preprint has been peer-reviewed and recommended by Peer Community In Zool (https://doi.org/10.24072/pci.zool.100003). The name of one reviewer has been added. * http://doi.org/10.5281/zenodo.3629451 * http://doi.org/doi:10.5281/zenodo.3901471
DOI:10.1101/653980