Advancing proteomics with ion/ion chemistry : Mass spectrometry for proteomics analysis
Mass spectrometers, instruments that use electric and/or magnetic fields to measure a gas-phase ion's mass-to-charge ratio (m/z), are used in a wide variety of applications—with the field having a reputation for providing good sensitivity and high-informing power. Protein analysis (proteomics)...
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Published in | BioTechniques Vol. 40; no. 6; pp. 783 - 789 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Natick, MA
Eaton
01.06.2006
Future Science Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | Mass spectrometers, instruments that use electric and/or magnetic fields to measure a gas-phase ion's mass-to-charge ratio (m/z), are used in a wide variety of applications—with the field having a reputation for providing good sensitivity and high-informing power. Protein analysis (proteomics) is a relatively recent affair for the field and was enabled in the late 1980s with the advent of biomolecule ionization methods such as electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI). Today, the area of protein analysis garners considerable attention from many in the mass spectrometry (MS) field; given the myriad of possible protein forms and their broad dynamic range (abundance) in the cell, the analytical challenge is paramount. Here we discuss a developing technology—ion/ion chemical reactions—that promises to transform how we think about and conduct protein sequence analysis via MS. |
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ISSN: | 0736-6205 1940-9818 |
DOI: | 10.2144/000112194 |