Real-time PCR analysis of trinucleotide repeat allele expansions in the androgen receptor gene

The expansion of specific trinucleotide repeats results in certain genetic disorders. Real-time PCR analysis was used to rapidly discriminate between normal and expanded (CAG)(n) alleles in the androgen receptor gene. The difference in melting temperature (T(m)) between the most common normal and ex...

Full description

Saved in:
Bibliographic Details
Published inMolecular diagnosis Vol. 9; no. 4; p. 217
Main Authors Chatzikyriakidou, Anthoula, Yapijakis, Christos, Sofikitis, Nikolaos, Vassilopoulos, Dimitrios, Georgiou, Ioannis
Format Journal Article
LanguageEnglish
Published United States 2005
Subjects
Alleles
Chromosomes, Human, X
Female
Humans
Male
Muscular Disorders, Atrophic - genetics
Receptors, Androgen - genetics
Transition Temperature
Trinucleotide Repeat Expansion
Online AccessGet more information

Cover

Abstract The expansion of specific trinucleotide repeats results in certain genetic disorders. Real-time PCR analysis was used to rapidly discriminate between normal and expanded (CAG)(n) alleles in the androgen receptor gene. The difference in melting temperature (T(m)) between the most common normal and expanded alleles was approximately 1 degrees C. Real-time PCR analysis seems to be a highly reliable and rapid method, which may facilitate the first molecular approach to human trinucleotide repeat disorders.
AbstractList The expansion of specific trinucleotide repeats results in certain genetic disorders. Real-time PCR analysis was used to rapidly discriminate between normal and expanded (CAG)(n) alleles in the androgen receptor gene. The difference in melting temperature (T(m)) between the most common normal and expanded alleles was approximately 1 degrees C. Real-time PCR analysis seems to be a highly reliable and rapid method, which may facilitate the first molecular approach to human trinucleotide repeat disorders.
Author Yapijakis, Christos
Vassilopoulos, Dimitrios
Georgiou, Ioannis
Chatzikyriakidou, Anthoula
Sofikitis, Nikolaos
Author_xml – sequence: 1
  givenname: Anthoula
  surname: Chatzikyriakidou
  fullname: Chatzikyriakidou, Anthoula
  organization: Genetics Unit, Department of Obstetrics and Gynaecology, Ioannina University School of Medicine, Ioannina, Greece
– sequence: 2
  givenname: Christos
  surname: Yapijakis
  fullname: Yapijakis, Christos
– sequence: 3
  givenname: Nikolaos
  surname: Sofikitis
  fullname: Sofikitis, Nikolaos
– sequence: 4
  givenname: Dimitrios
  surname: Vassilopoulos
  fullname: Vassilopoulos, Dimitrios
– sequence: 5
  givenname: Ioannis
  surname: Georgiou
  fullname: Georgiou, Ioannis
BackLink https://www.ncbi.nlm.nih.gov/pubmed/16392902$$D View this record in MEDLINE/PubMed
BookMark eNo1j81Kw0AUhWdRsT-68QFkXiB65y-ZLLXYKhSUolvLTXKjI5NJmEnBvr0BdXU48J0PzpLNQh-IsSsBNwKguL3fgJI5QGlmbCHA6syaUs7ZMqUvACFMqc7ZXOSqlCXIBXvfE_psdB3xl_WeY0B_Si7xvuVjdOFYe-pH1xCPNBCOHL0nT5y-BwzJ9SFxF_j4SdOyif0HhQmsaRj7yKdCF-ysRZ_o8i9X7G3z8Lp-zHbP26f13S6rhQKZGavbWtg2L-oCUKI1JpcaNVaVbkRV5FCVRlHeWkHKgq5RqaKRFaEGQGXkil3_eodj1VFzGKLrMJ4O_0flD3kkVD4
CitedBy_id crossref_primary_10_1007_s12035_021_02314_z
ContentType Journal Article
DBID CGR
CUY
CVF
ECM
EIF
NPM
DOI 10.1007/BF03260095
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
DatabaseTitleList MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod no_fulltext_linktorsrc
Discipline Medicine
Biology
ExternalDocumentID 16392902
Genre Journal Article
GroupedDBID --K
0R~
123
1B1
1RT
1~5
29M
4.4
4G.
53G
5VS
6I2
7-5
95.
AAEDT
AAKAS
AALRI
AAQFI
AAQXK
AAXUO
ABFTV
ABJNI
ABJOX
ABKCH
ABTMW
ACGFS
ADMUD
ADURQ
ADZCM
AEOHA
AFTJW
AGDGC
AGQMX
AITUG
ALMA_UNASSIGNED_HOLDINGS
AMKLP
ASPBG
AVWKF
AZFZN
A~4
BGNMA
CAG
CGR
COF
CS3
CUY
CVF
EBS
ECM
EIF
EJD
EO8
EO9
EP2
F5P
FDB
FEDTE
FGOYB
G-Q
HVGLF
HZ~
IHE
M41
M4Y
NPM
NQ-
NQJWS
NU0
O9-
OAC
OVD
R2-
ROL
RPZ
RSV
RZALA
SDG
SEW
SOJ
SSZ
TEORI
UG4
UHS
YQY
~JE
ID FETCH-LOGICAL-c1302-584fc18f67c70a2a855624a4abb4d1b760b953e6f81e3804ca337d2bea400a352
ISSN 1084-8592
IngestDate Sat Sep 28 07:52:34 EDT 2024
IsPeerReviewed true
IsScholarly true
Issue 4
Language English
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c1302-584fc18f67c70a2a855624a4abb4d1b760b953e6f81e3804ca337d2bea400a352
PMID 16392902
ParticipantIDs pubmed_primary_16392902
PublicationCentury 2000
PublicationDate 2005-00-00
PublicationDateYYYYMMDD 2005-01-01
PublicationDate_xml – year: 2005
  text: 2005-00-00
PublicationDecade 2000
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Molecular diagnosis
PublicationTitleAlternate Mol Diagn
PublicationYear 2005
SSID ssj0011593
Score 1.600914
Snippet The expansion of specific trinucleotide repeats results in certain genetic disorders. Real-time PCR analysis was used to rapidly discriminate between normal...
SourceID pubmed
SourceType Index Database
StartPage 217
SubjectTerms Alleles
Chromosomes, Human, X
Female
Humans
Male
Muscular Disorders, Atrophic - genetics
Receptors, Androgen - genetics
Transition Temperature
Trinucleotide Repeat Expansion
Title Real-time PCR analysis of trinucleotide repeat allele expansions in the androgen receptor gene
URI https://www.ncbi.nlm.nih.gov/pubmed/16392902
Volume 9
hasFullText
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1dT9swFLXK0CZeJihs41N-4K0KSlMncR5R2VQhFaFCEbwM2YkjvHZJVKUP9Ifs9-7eOkkTBOLjJYriyml8j6-P7etzCTlmkbIDEUSWJ4VtsYA5FvBkPAUCXS_mSpkd3eGFNxiz81v3ttX6V4tamufyJFw8e67kI1aFZ2BXPCX7DstWlcIDuAf7whUsDNc32XgELM_C7PCdy_6oI2r6IvlMJ6hUnOY6wsQoGS4EYNqUqUJRfxifyhByJJ4CVQvgLfBDjHJJZ5hYuREjNCyz6OJiLcbm6YqL9x9EvtCTR_jeiY7SeSVJMJ9WLv9OZPoPctWankFVwRW0wQSllQwyJzDZXhXeALnX0zSD2kxA4BmeyJrptLle4dbWK4yLtTmzuBs0fHBQgxpr-FP_WT9vV9HrKLBv0nTWDJ79XVocuCawv-WJ7ldKn2hul0VrZM3n6D0vcA2o2JvqGinn6juaorfFH0Ix2qKSJxOWJXG53iRfixkHPTXw2SItlbTJZ5OD9LFNvgyL6Ipt8rvCEwU80RJPNI1pA0_U4IkaPNEVnqhOKOCJlniiJZ4o4mmHjH_9vO4PrCL_hhXidrYF3DQOuzz2_NC3hSO4C2SZCSakZFFX-p4tA7enoEt3VY_bLBS9nh85UgkYGAQw-2_kU5Im6gehXqjcSLhOoOyQ8VAIxrh0Ixg8eOAp6eyS76aN7jMjsnJftt7eiyX7ZGOFrwOyHkOvVodAEXN5tLTYf-ClaO8
link.rule.ids 783
linkProvider National Library of Medicine
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Real-time+PCR+analysis+of+trinucleotide+repeat+allele+expansions+in+the+androgen+receptor+gene&rft.jtitle=Molecular+diagnosis&rft.au=Chatzikyriakidou%2C+Anthoula&rft.au=Yapijakis%2C+Christos&rft.au=Sofikitis%2C+Nikolaos&rft.au=Vassilopoulos%2C+Dimitrios&rft.date=2005-01-01&rft.issn=1084-8592&rft.volume=9&rft.issue=4&rft.spage=217&rft_id=info:doi/10.1007%2FBF03260095&rft_id=info%3Apmid%2F16392902&rft_id=info%3Apmid%2F16392902&rft.externalDocID=16392902
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1084-8592&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1084-8592&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1084-8592&client=summon