Real-time PCR analysis of trinucleotide repeat allele expansions in the androgen receptor gene

The expansion of specific trinucleotide repeats results in certain genetic disorders. Real-time PCR analysis was used to rapidly discriminate between normal and expanded (CAG)(n) alleles in the androgen receptor gene. The difference in melting temperature (T(m)) between the most common normal and ex...

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Bibliographic Details
Published inMolecular diagnosis Vol. 9; no. 4; p. 217
Main Authors Chatzikyriakidou, Anthoula, Yapijakis, Christos, Sofikitis, Nikolaos, Vassilopoulos, Dimitrios, Georgiou, Ioannis
Format Journal Article
LanguageEnglish
Published United States 2005
Subjects
Alleles
Chromosomes, Human, X
Female
Humans
Male
Muscular Disorders, Atrophic - genetics
Receptors, Androgen - genetics
Transition Temperature
Trinucleotide Repeat Expansion
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Summary:The expansion of specific trinucleotide repeats results in certain genetic disorders. Real-time PCR analysis was used to rapidly discriminate between normal and expanded (CAG)(n) alleles in the androgen receptor gene. The difference in melting temperature (T(m)) between the most common normal and expanded alleles was approximately 1 degrees C. Real-time PCR analysis seems to be a highly reliable and rapid method, which may facilitate the first molecular approach to human trinucleotide repeat disorders.
ISSN:1084-8592
DOI:10.1007/BF03260095