Critical periods during development of the dentate nucleus and their clinical significance

• Published in: Medicinski pregled Vol. 60; no. 11-12; pp. 537 - 541
• Main Authors: Gudović, Radmila, Mijatov-Ukropina, Ljilja, Stojsić-Dzunja, Ljubica, Marić, Dusica, Babović, Sinisa S, Krstonosić, Biljana
• Format: Journal Article
• Language: Serbian; English
• Published: Serbia 01.11.2007
• Subjects: Cerebellar Nuclei - embryology; Gestational Age; Humans
• ISSN: 0025-8105; 1820-7383
• DOI: 10.2298/MPNS0712537G

The aim of this study was to identify the critical periods in the development of the human dentate nucleus in fetuses of different gestational ages and in one newborn brain. The fetal brains were fixed in alcohol-formalin-acetic acid, embedded in paraffin, cut into 30 micro sections, and stained with cresyl violet. The sections were examined by light microscopy. In order to identify vulnerable periods, histological and stereological analyses were done. FORMATION OF THE DENTATE NUCLEUS: The first appearance of the dentate nucleus was noticed in fetus of 12.5 weeks of gestation (wg), and its cells corresponded to the first and second stage of maturation. Formation of the dorsomedial lamina begins at the end of the 13th wg, and it starts to fold at 19.5 wg. At this time, cells correspond to the third stage of maturation, and formation of the ventromedial lamina begins. The first folds of the ventromedial lamina are noticed at 23.5 wg. Fourth stage maturity cells are noticed at 23.5 wg. remaining conspicuous up to birth. The numerical density of the nerve cell nuclei shows a constant decrease. Based on our results, we can conclude that during development of the dentate nucleus, there are two vulnerable periods. The first one corresponds to the fourth month of intrauterine life, and the second to the intensive growth of the dorsomedial and ventrolateral lamina (20.0 - 24.5 wg).