Front Cover: Chemoenzymatic Labeling to Visualize Intercellular Contacts Using Lipidated SortaseA (ChemBioChem 21/2022)

A simple and versatile strategy is introduced for the selective fluorescent labeling of intercellular contacting cells. A transpeptidase sortase A and its substrate triglycine peptide (GGGYC sequence) were chemically conjugated with a lipidated polymer and then anchored onto the membranes of differe...

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Bibliographic Details
Published inChembiochem : a European journal of chemical biology Vol. 23; no. 21
Main Authors Yamaguchi, Satoshi, Ikeda, Ryosuke, Umeda, Yuki, Kosaka, Takahiro, Yamahira, Shinya, Okamoto, Akimitsu
Format Journal Article
LanguageEnglish
Published 04.11.2022
Subjects
cell recognition
cell-cell communication
enzymes
fluorescent labeling
protein lipidation
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Summary:A simple and versatile strategy is introduced for the selective fluorescent labeling of intercellular contacting cells. A transpeptidase sortase A and its substrate triglycine peptide (GGGYC sequence) were chemically conjugated with a lipidated polymer and then anchored onto the membranes of different cells. The triglycine modified cells (displayed in red) were labeled with a fluorescent dye by contacting with the sortase A‐modified cells based on proximity‐dependent transpeptidation with a dye‐modified labeling peptide (DLPETGG sequence). More information can be found in the Research Article by S. Yamaguchi, A. Okamoto et al.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.202200567