Establishment of Rapid Propagation System for Saponaria officinalis L. Progeny After EMS Mutation

Saponaria officinalis L. seeds were treated with different concentrations of chemical mutagen to determine the impact of media with various hormone concentrations on the induction of mutated （Saponaria officinalis L.） seeds＇ adventitious buds and rooting culture by using tissue culture technology. T...

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Bibliographic Details
Published inThe journal of Northeast Agricultural University Vol. 19; no. 1; pp. 21 - 26
Main Authors Xue, Yang, Jin-gang, Wang, Tao, Yang, Shan-shan, Li, Hong-wei, Ren, Guo-hua, Fu, Shu-fang, Gong
Format Journal Article
LanguageEnglish
Published Elsevier (Singapore) Pte Ltd 01.03.2012
Subjects
chemical mutagen
Saponaria officinalis L
tissue culture
Saponaria officinalis L
chemical mutagen
tissue culture
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Summary:Saponaria officinalis L. seeds were treated with different concentrations of chemical mutagen to determine the impact of media with various hormone concentrations on the induction of mutated （Saponaria officinalis L.） seeds＇ adventitious buds and rooting culture by using tissue culture technology. The results indicated that the optimum concentration of the chemical mutagen-EMS was 0.3%; the optimum induction medium was MS＋6-BA 1.5 mg · L^-1, NAA 0.1 mg · L^-1 and the optimum rooting medium was MS＋0.05 mg · L^-1 NAA.
Bibliography:Saponaria officinalis L., chemical mutagen, tissue culture
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Saponaria officinalis L. seeds were treated with different concentrations of chemical mutagen to determine the impact of media with various hormone concentrations on the induction of mutated （Saponaria officinalis L.） seeds＇ adventitious buds and rooting culture by using tissue culture technology. The results indicated that the optimum concentration of the chemical mutagen-EMS was 0.3%; the optimum induction medium was MS＋6-BA 1.5 mg · L^-1, NAA 0.1 mg · L^-1 and the optimum rooting medium was MS＋0.05 mg · L^-1 NAA.
ISSN:1006-8104
DOI:10.1016/S1006-8104(12)60034-8