The role of microbiome in uveitis in experimental mice model

Unlike the individual's genetic makeup, gut microbiota can be influenced, and thus used in the disease therapy or even prevention. We have shown that absence or reduction of microbiota modifies the inflammatory response in the murine model of experimental autoimmune uveoretinitis (EAU). Our fur...

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Bibliographic Details
Published inActa ophthalmologica (Oxford, England) Vol. 100; no. S275
Main Authors Heissigerova, Jarmila, Dusek, Otakar, Fajstova, Alena, Klimova, Aneta, Brichova, Michaela, Svozilkova, Petra, Kverka, Miloslav
Format Journal Article
LanguageEnglish
Published Malden Wiley Subscription Services, Inc 01.12.2022
Subjects
Animal models
E coli
Escherichia coli
Experimental autoimmune uveitis
Experimental autoimmune uveoretinitis
Freund's adjuvant
Inflammation
Interphotoreceptor retinoid-binding protein
Intestinal microflora
Microbiomes
Microbiota
Oral administration
Pertussis
Pertussis toxin
Probiotics
Statistical analysis
Uveitis
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Summary:Unlike the individual's genetic makeup, gut microbiota can be influenced, and thus used in the disease therapy or even prevention. We have shown that absence or reduction of microbiota modifies the inflammatory response in the murine model of experimental autoimmune uveoretinitis (EAU). Our further purpose of the project was to test influence of probiotics on intensity of intraocular inflammation in EAU. Uveitis was induced in C57BL/6J mice by interphotoreceptor retinoid binding protein in complete Freund's adjuvant and with pertussis toxin. The grade of uveitis was assessed in vivo by using otoscope connected to camera and post mortem on haematoxylin and eosin stained tissues. Bacteria Escherichia coli were administered orally three times a week and saline solution was administered to control group. Different regimens of administration were tested. Interestingly, mice administered with probiotics reached statistically significant lower grade of uveitis in comparison to the control group in clinical and histological evaluation. This may have valuable implications for the control of sight‐threatening uveitis in patients. Supported by research project AZV MZ CR 17‐31248A. Key words: microbiome, probiotics, Escherichia coli, experimental autoimmune uveitis, EAU.
ISSN:1755-375X
1755-3768
DOI:10.1111/j.1755-3768.2022.15460