1,25-dihydroxyvitamin D 3 ameliorates high glucose-mediated proliferation, migration, and MCP-1 secretion of vascular smooth muscle cells by inhibiting MAPK phosphorylation
Objectives To explore the impacts of 1,25-dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ) on the proliferation, migration, and monocyte chemoattractant protein-1 (MCP-1) secretion of vascular smooth muscle cells (VSMCs) in a high glucose environment and its possible mechanism. Methods We extracted VSMCs from...
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Published in | Journal of international medical research Vol. 50; no. 9; p. 30006052211219 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
01.09.2022
|
Online Access | Get full text |
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Summary: | Objectives
To explore the impacts of 1,25-dihydroxyvitamin D
3
(1,25(OH)
2
D
3
) on the proliferation, migration, and monocyte chemoattractant protein-1 (MCP-1) secretion of vascular smooth muscle cells (VSMCs) in a high glucose environment and its possible mechanism.
Methods
We extracted VSMCs from the thoracic aorta of a male Sprague–Dawley rats before culturing them in a 25-mM glucose-containing medium in the presence or absence of 1,25(OH)
2
D
3
(10
−9
–10
−7
M). Cell proliferation was determined by bromodeoxyuridine incorporation assays. Subsequently, cell migratory capacity was examined by performing a transwell assay. An enzyme-linked immunosorbent assay was conducted to assess MCP-1 levels. Protein levels of matrix metalloproteinase-9 (MMP-9), mitogen-activated protein kinases (MAPKs), cyclin D1, and phosphorylated MAPKs were determined by immunoblotting.
Results
1,25(OH)
2
D
3
significantly suppressed the proliferation, migration, and MCP-1 secretion of VSMCs mediated by high glucose in a dose-dependent manner, diminished the enhanced protein expression of MMP-9 and cyclin D1, and attenuated MAPK phosphorylation. The p38 inhibitor SB203580 and ERK1/2 inhibitor PD98059 suppressed high glucose-mediated upregulation of MMP-9 and cyclin D1 protein expression and MCP-1 secretion, respectively.
Conclusions
1,25(OH)
2
D
3
ameliorates high glucose-mediated proliferation, migration, and MCP-1 secretion of VSMCs by inhibiting MAPK phosphorylation, implying a potential therapeutic approach using 1,25(OH)
2
D
3
for diabetic macrovascular complications. |
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ISSN: | 0300-0605 1473-2300 |
DOI: | 10.1177/03000605221121973 |