Development of a technique for measuring the oxidation rate of a 14C-labelled substrate from 14CO2 production without the need for collection of expired air

1. A method is proposed for the determination of the rate of oxidation of 14C-labelled substrates in man from blood sampling only. 2. The method was verified by simulating release of 14C into the bicarbonate pool of patients by using a constant-rate infusion of sodium [14C]bicarbonate, the plasma le...

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Published inClinical science (1979) Vol. 60; no. 2; pp. 233 - 235
Main Authors Clague, M B, Keir, M J, Wright, P D
Format Journal Article
LanguageEnglish
Published England 01.02.1981
Subjects
Bicarbonates - metabolism
Carbon Dioxide - blood
Carbon Radioisotopes
Humans
Methods
Oxidation-Reduction
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Summary:1. A method is proposed for the determination of the rate of oxidation of 14C-labelled substrates in man from blood sampling only. 2. The method was verified by simulating release of 14C into the bicarbonate pool of patients by using a constant-rate infusion of sodium [14C]bicarbonate, the plasma level of total 14CO2 (d.p.m. ml-1 70 kg-1) correlating well (r = 0.878, P = 0.001) with the infusion rate (microCi/h). 3. Techniques involved in employing this method are discussed, particularly in the light of errors involved.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0143-5221
1470-8736
DOI:10.1042/cs0600233