Chemoenzymatic elaboration of monosaccharides using engineered cytochrome P450 BM3 demethylases
Polysaccharides comprise an extremely important class of biopolymers that play critical roles in a wide range of biological processes, but the synthesis of these compounds is challenging because of their complex structures. We have developed a chemoenzymatic method for regioselective deprotection of...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 106; no. 39; pp. 16550 - 16555 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
29.09.2009
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Online Access | Get full text |
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Summary: | Polysaccharides comprise an extremely important class of biopolymers that play critical roles in a wide range of biological processes, but the synthesis of these compounds is challenging because of their complex structures. We have developed a chemoenzymatic method for regioselective deprotection of monosaccharide substrates using engineered
Bacillus megaterium
cytochrome P450 (P450
BM3
) demethylases that provides a highly efficient means to access valuable intermediates, which can be converted to a wide range of substituted monosaccharides and polysaccharides. Demethylases displaying high levels of regioselectivity toward a number of protected monosaccharides were identified using a combination of protein and substrate engineering, suggesting that this approach ultimately could be used in the synthesis of a wide range of substituted mono- and polysaccharides for studies in chemistry, biology, and medicine. |
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ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.0908954106 |