Genetic diversity analysis and phylogeography of Osteochilus spilurus (Cyprinidae: Labeoninae) from Bangka, Belitung, and Kalimantan Islands using Cytochrome b gene

• Published in: Biodiversitas (Surakarta) Vol. 23; no. 9
• Main Authors: Kurniawan, Ardiansyah, Kurniawan, Andi, Hariati, Anik Martinah, Kurniawan, Nia, Nugroho, Teguh W, Bidayani, Endang, Syarif, Ahmad F, Wiadnya, Dewa Gede Raka
• Format: Journal Article
• Language: English
• Published: 24.09.2022
• ISSN: 1412-033X; 2085-4722
• DOI: 10.13057/biodiv/d230941

Kurniawan A, Hariati AM, Kurniawan A, Kurniawan N, Nugroho TW, Bidayani E, Syarif AF, wiadnya DGR. 2022. Genetic diversity analysis and phylogeography of Osteochilus spilurus (Cyprinidae: Labeoninae) from Bangka, Belitung, and Kalimantan Islands using Cytochrome b gene. Biodiversitas 23: 4738-4746. The Sundaland fish species, Osteochilus spilurus is only utilized commercially in the Belitung Islands and has yet to be domesticated. This species can characterize freshwater fish phylogeography because have a minimum of human influence. This study aimed to analyze the genetic diversity and phylogeography of O. spilurus from the Bangka, Belitung, and Kalimantan Islands using Cytochrome b gene. Twenty fish samples were collected from the Sebangau and Kapuas Rivers in Kalimantan, the Lenggang River in Belitung Island, and the Lebak River in Bangka Island. Forward LA-cyp and reverse HA-cyp primers were used for amplification Cytochrome b gene. Mega 10.0 applications were used to construct of the phylogenetic tree. The phylogeographic analysis uses a comparative phylogenetic tree approach with geographical history. The cytochrome b gene showed variations in the nucleotides of O. spilurus from the three islands. Phylogeography of O. spilurus showed that there are two different clades with intraspecific variation according to a geographic area with bootstrap values of 99-100%. One clade contains fish samples from Bangka and Belitung islands, while the other clade comes from Kalimantan island. The formation of the Karimata Strait (between Bangka-Belitung and Kalimantan Islands) and the pattern of paleo rivers are predicted to influence the disparity.