Influence on the immune function of the human peripheral blood mononuclear cells transfected by retrovirusmediated HSV-tk gene

• Published in: Chinese medical journal Vol. 119; no. 12; pp. 1038 - 1041
• Main Authors: XUE, Xing-kui, SUN, Wen-ji, HU, Jie, YAO, Hang-ping, CAI, Zhen
• Format: Journal Article
• Language: English
• Published: 01.06.2006
• Subjects: 免疫功能; 单核细胞; 基因表达; 外周血
• ISSN: 0366-6999; 2542-5641
• DOI: 10.1097/00029330-200606020-00013

Graft-versus-host disease （GVHD） is a severe complication and a major source of morbidity and mortality after allogeneic hematopoietic stem cell transplantation （HSCT）. Although T cell depletion of the allogeneic HSCT can efficiently prevent GVHD, it is associated with increased graft rejection and relapse of the malignant disease. To preserve the beneficial effects of donor T cells and avoid their GVHD effects, some approaches have been explored. One of them is to transfer a special suicide gene into the donor T lymphocytes so that they become more sensitive to a specific drug that is ordinarily not toxic. The most commonly used suicide gene is the thymidine kinase-encoding gene of herpes simplex virus （HSV-tk）.