In vivo insulin effect on ATPase activities in erythrocyte membrane from insulin-dependent diabetics

• Published in: Diabetes (New York, N.Y.) Vol. 36; no. 9; pp. 991 - 995
• Main Authors: D Rahmani-Jourdheuil, Y Mourayre, P Vague, J Boyer, I Juhan-Vague
• Format: Journal Article
• Language: English
• Published: American Diabetes Association 01.09.1987
• ISSN: 0012-1797; 1939-327X; 0012-1797
• DOI: 10.2337/diabetes.36.9.991

In vivo insulin effect on ATPase activities in erythrocyte membrane from insulin-dependent diabetics. D Rahmani-Jourdheuil , Y Mourayre , P Vague , J Boyer and I Juhan-Vague Abstract Na+-K+-dependent ouabain-sensitive ATPase and Mg2+-ATPase have been assayed in the erythrocyte membranes of control subjects and in uncontrolled type I (insulin-dependent) diabetics. A decrease in Na+-K+-ATPase activity was observed in the patients that was significantly correlated with glycemia. The Mg2+-ATPase was increased moderately, and no correlation with glycemia was found. To study the in vivo effect of insulin, ATPase activities were measured in uncontrolled diabetics before and after a 24-h continuous insulin perfusion administered by means of an artificial pancreas. ATPase activities were corrected after normalization of glycemia. It therefore seems that glycemia and/or insulinemia are involved in the regulation of erythrocyte Na+-K+ ouabain-sensitive ATPase and to a lesser extent in that of Mg2+-dependent ATPase.