Characterization and antimicrobial resistance of Moraxella ovis isolates from clinical cases of contagious ovine keratoconjunctivitis in Mexico State, Mexico

• Published in: Revista Colombiana de Ciencias pecuarias Vol. 37; no. 1; pp. 14 - 26
• Main Authors: Salgado Miranda, Celene, Soriano Vargas E., Edgardo, Acosta, Jorge, Talavera Rojas, Martín, Enríquez Gómez, Edgar, Palomares Reséndiz, Erika G, Ortiz Arana, Giovany
• Format: Journal Article
• Language: English
• Published: Medellín Universidad de Antioquía 01.01.2024
• Subjects: Antimicrobial agents; Antimicrobial resistance; cabra; ceratoconjuntivite contagiosa ovina; contagious ovine keratoconjunctivitis; Disease resistance; Drug resistance; epidemiological surveillance; Epidemiology; Eye diseases; Genes; genes de resistencia; genes de resistência; goat; Keratoconjunctivitis; Moraxella; Moraxella ovis; Moraxella spp; multiresistance; multirresistencia; multirresistência; oveja; ovelha; queratoconjuntivitis contagiosa ovina; resistance genes; resistencia antimicrobiana; resistência antimicrobiana; rRNA 16S; Sheep; vigilancia epidemiológica; vigilância epidemiológica; Mexico
• ISSN: 0120-0690; 2256-2958
• DOI: 10.17533/udea.rccp.v37n1a4

Background: Contagious ovine keratoconjunctivitis (OKC) causes blindness in sheep and goats and it is associated with a set of bacterial genera of which some species show antimicrobial resistance. Objective: To identify phenotypicgenotypic relationship of antimicrobial resistance from Moraxellaspp. isolates obtained from clinical cases of contagious ovine keratoconjunctivitis (OKC) in Mexico. Methods: A total of 209 samples were obtained from clinical cases of OKC in sheep and 60 Moraxella ovisisolates were identified by bacteriological techniques and amplification of 16s rRNAand rtxAgenes by PCR. All isolates were evaluated in terms of antimicrobial resistance by the disk diffusion susceptibility test and amplification of resistance genes by PCR. Results: We found 14 Moraxella ovisisolates with antimicrobial resistance (AMR) and five multiresistant (MDR). The sul1, sul2, tetB, qnrA, qnrB, BlaTEM genes of antimicrobial resistance were amplified, while gene floRwas not amplified. Conclusion: This is the first isolation report of Moraxella ovisfrom ocular lesions in sheep in the State of Mexico, with six antimicrobial resistance genes identified. Results suggest that Moraxella ovisplays an important role in the course of the disease and provides a panorama for molecular epidemiological surveillance and bacterial resistance.