A PCR-Aided Transcript Titration Assay Revealing Very Low Expression of a Gene at Band 3p21 in 33 Cell Lines Derived from all Types of Lung Cancer

We have developed a general PCR-based method to quantify the amount of a specific mRNA present in a given cell line or tissue. We applied this quantitative PCR to analyse the expression of D8, a human gene which we recently identified in the chromosomal region 3p21, the common deletion region of lun...

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Bibliographic Details
Published inEuropean journal of human genetics : EJHG Vol. 1; no. 2; pp. 156 - 163
Main Authors Kok, Klaas, van den Berg, Anke, Buchhagen, Dorothy L., Carritt, Ben, Buys, Charles H.C.M
Format Journal Article
LanguageEnglish
Published Basel, Switzerland 01.02.1993
Subjects
Original Paper
Chromosome 3
Gene expression
Lung cancer
Quantitative PCR
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Summary:We have developed a general PCR-based method to quantify the amount of a specific mRNA present in a given cell line or tissue. We applied this quantitative PCR to analyse the expression of D8, a human gene which we recently identified in the chromosomal region 3p21, the common deletion region of lung cancer. Our PCR-aided assay shows that in most lungcancer- derived cell lines the amount of D8 transcripts is only 2% or less of that in normal lung tissue. The virtual absence of expression may imply some role of the gene in the development of lung cancer.
ISSN:1018-4813
1476-5438
DOI:10.1159/000472402