Investigation Of Q Fever İn Kilis And Shamil Goats By ELISA And Touchdown-PCR

This study was carried out on Kilis and Shamil goats to determine the different stages of Q fever-chronic and acute- in serum samples by indirect ELISA and in blood samples by touchdown-PCR, respectively. A total of 92 serum samples comprising 46 Kilis and 46 Shamil goats was examined and 7.60%, 4.3...

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Bibliographic Details
Published inVeteriner Fakültesi dergisi Vol. 61; no. 3
Main Authors Dalkılıç,Bestami, Günaydın,Elçin, Pekkaya,Selçuk, Müştak,H. Kaan
Format Journal Article
LanguageEnglish
Published Ankara Üniversitesi Veteriner Fakültesi 01.03.2014
Subjects
Sağlık Hizmetleri
Tıp
Ankara
Türkiye
goat
Q fever
Q humması
keçi
ELISA
touchdown-PCR
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Summary:This study was carried out on Kilis and Shamil goats to determine the different stages of Q fever-chronic and acute- in serum samples by indirect ELISA and in blood samples by touchdown-PCR, respectively. A total of 92 serum samples comprising 46 Kilis and 46 Shamil goats was examined and 7.60%, 4.34% and 10.8% seropositivity were determined for total serum samples, Kilis and Shamil goat serum samples, respectively. Of the examined 92 blood samples belonging to 46 Kilis and 46 Shamil goats, none of them yielded 687 bp PCR products by touchdown-PCR. The positive ELISA titers were thought to be the evidence of previous infection. The province of Kilis localized in South-East Anatolian Region of Turkey was decided to be a risky region for Q fever.
ISSN:1300-0861
1308-2817
DOI:10.1501/Vetfak_0000002623