Inhibition of lipid peroxidation as prognostic biomarkers of wound healing

• Published in: Planta Medica
• Main Authors: Odukoya, OA, Sofidiya, MO, Ajose, OI, Onalo, MU, Shuaib, SA
• Format: Conference Proceeding
• Language: English
• Published: 21.07.2009
• ISSN: 0032-0943; 1439-0221
• DOI: 10.1055/s-0029-1234509

Molecular oxygen plays a central role in the pathogenesis and therapy of wounds. Ethanol extracts of six wound healing medicinal plants ( Anthocleista nobilis G. Don (Loganiaceae), Entandrophragma utile (Dawe & Sprague) Meliaceae, Nauclea latifolia (Rubiaceae) Petiveria alliaceae (Phytolaccaceae), Treculia africana Dec'ne (Moraceae) and Uvaria chamae P. Beauv. (Annonaceae) identified in an ethnobotanical survey were investigated for free radical scavenging activities and also lipid peroxidation. Free radical scavenging activity was evaluated using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radicals and inhibition of lipid peroxidation was accessed with thiobarbituric acid (TBA) method in a poly unsaturated fatty acid (PUFA) model of Scomber japonicum fish homogenate calculated as MDA equivalent/g of tissue. Total phenol and flavonoid contents were determined spectrophotometrically as gallic acid and rutin equivalents, respectively . Plant Samples Thiobarbituric Acid Reactive Substances (TBARS) Values (mg/tissue) in fish homogenates Total Phenols Total Flavonoid Raw Cooked A. nobilis 0.009±0.006 0.007±0.006 10.020±0.016 1.145±0.015 E. utile 0.010±0.001 0.005±0.002 30.101±0.089 17.000±0.008 N. latifolia 0.008±0.002 0.009±0.000 10.411±0.153 15.192±0.006 P. alliaceae 0.005±0.001 0.006±0.000 3.133±0.040 17.474±0.005 T. africana 0.006±0.002 0.004±0.000 3.620±0.020 0.189±0.000 U.chamae 0.017±0.001 0.007±0.000 11.903±0.030 12.233±0.020 Flavonoid content correlated positively with activity. Flavonoids reduce lipid peroxidation by preventing or slowing the onset of cell necrosis and also by improving vascularity. Hence, any extract that inhibits lipid peroxidation will increase the viability of collagen fibrils by increasing the strength of collagen fibres, circulation, prevent cell damage and hasten the process of wound healing by inhibition of lipid peroxidation as prognostic biomarkers.