Three anti-inflammatory stigmastane steroids from Alchornea floribunda leaves
The anti-inflammatory activity of Alchornea cordifolia has been reported in previous studies [1,2,3]. In the present study, bioactivity – guided fractionation led to the isolation of three stigmastane steroids from Alchornea floribunda leaves. The anti-inflammatory activities of these compounds were...
Saved in:
Published in | Planta Medica |
---|---|
Main Authors | , , , , |
Format | Conference Proceeding |
Language | English |
Published |
04.08.2008
|
Online Access | Get full text |
Cover
Loading…
Summary: | The anti-inflammatory activity of
Alchornea cordifolia
has been reported in previous studies [1,2,3]. In the present study, bioactivity – guided fractionation led to the isolation of three stigmastane steroids from
Alchornea floribunda
leaves. The anti-inflammatory activities of these compounds were evaluated using in vitro and in vivo animal models. The compounds,
1
,
2
and
3
at 5mg/ear inhibited xylene – induced ear edema in mice, with edema inhibitions of 59.9, 51.5 and 51.54% respectively. At 20mg/kg (ip), all the compounds significantly (p<0.05) inhibited acute inflammation induced by subplanta injection of undiluted egg albumen in rats' paw. Compound
1
exhibited a higher anti-inflammatory effect (% edema inhibition=50. 0) than prednisolone (% edema inhibition=48.0) at 3h.
1
and
3
at concentrations of 50 and 100µg/ml significantly (p<0.05) stabilized heat-induced haemolysis of human erythrocytes
in vitro
, but have no effect on hypotonicity-induced haemolysis. Spectral analysis elucidated the compounds as stigmastane –3, 6– dione (
1
), stigmastane –22– ene –3, 6– dione (
2
) and 3– hydroxystigmastane –22– ene (
3
). These compounds may, in part, account for the anti-inflammatory effect of
Alchornea floribunda
leaves. This is the first report on the isolation and structure elucidation of anti-inflammatory steroids from
Alchornea floribunda
leaves.
Acknowledgements: Institute of Anorganic Chemistry and Structure Chemistry, Heinrich-Heine-Universität, Germany. DrRuAngelie Edrada-Ebel Strathclyde Institute of Pharmacy and Biomedical Sciences University of Strathclyde Glasgow, Scotland.
References: 1. Osadebe, PO., Okoye, FBC. (2003)J Ethnopharmacol 89:19–24. 2. Manga et al. (2004). J Ethnopharmacol 92:209–214. 3. Osadebe et al. (2008). RPMP 22:589–595. |
---|---|
ISSN: | 0032-0943 1439-0221 |
DOI: | 10.1055/s-0028-1084177 |