Effect of Follicular Fluid Estradiol Level on Oocyte Quality, Fertilization and Pregnancy Outcome in Intracytoplasmic Sperm Injection Cycles

• Published in: QJM : An International Journal of Medicine Vol. 114; no. Supplement_1
• Main Authors: Al-Kady, Mohamed A, Mostafa, Mohamed H, Mansour, Dina Y, Mohamed, Noha R, Ahmed, Heba A, Rushdy, Emad E
• Format: Journal Article
• Language: English
• Published: Oxford University Press 01.10.2021
• Subjects: ICSI; pregnancy outcome; fertilization; oocyte quality; follicular fluid; estradiol
• ISSN: 1460-2725; 1460-2393
• DOI: 10.1093/qjmed/hcab115.016

Abstract Background Infertility is the third most serious disease worldwide estimated by the WHO. Despite the worldwide use of ICSI, it still has a low birth rate (30%). Patients and Methods The current study was a prospective study that included 180 women undergoing Intra-cytoplasmic sperm injection (ICSI) procedure. Using Flexible Antagonist protocol; during oocyte retrieval, follicular fluids of mature follicles (>17 mm) aspirated. Results The number of retrieved oocytes ranged from (1 to 33 oocytes) mean number 10±7. Follicular fluid E2 concentration ranged from 246±199 (0 to 700) (ng/ml). Oocyte maturation about 116 were MII (64.4%) of oocytes. And 64 (35.6%) were MI and GV. 107 cases (59.4) had normal fertilization lamos_zzzz@hotmail.com and 73(40%) had abnormal fertilization of MII injected oocytes. Embryo quality 63.9% (115) of fertilized MII oocytes were blastocysts and 65 (blastomere and others) and 60.6% of cases had embryo transfer on day 5 and 39.4 had transfer on day 3. Chemical pregnancy was positive in 85 cases (47.2%) and the clinical pregnancy positive in 66 cases (%). Conclusion Follicular fluid E2 concentration had fair predictive value in oocyte maturation, fertilization, embryo quality, chemical and clinical pregnancy. But it was an independent predictor of MII-grade oocytes production.