Unique Red Blood Cell Morphology Detected in a Patient with Myelodysplastic Syndrome by Three-dimensional Refractive Index Tomography

• Published in: Laboratory Medicine Online Vol. 9; no. 3; pp. 185 - 188
• Main Authors: Koo, Se-eun, Jang, Seongsoo, Park, Chan Jeoung, Cho, Young-Uk, Park, YongKeun
• Format: Journal Article
• Language: English
• Published: 대한진단검사의학회 01.07.2019
• Subjects: 임상병리학
• ISSN: 2093-6338; 2093-6338
• DOI: 10.3343/lmo.2019.9.3.185

The three-dimensional (3-D) shape of erythrocytes is strongly associated with various diseases. However, conventional optical imaging approaches with Wright's staining only provide information on two-dimensional morphology. Here, we employed optical diffraction tomography (ODT), a label-free 3-D quantitative phase imaging technique, and observed uniquely shaped red blood cells (RBCs) in the peripheral blood of a patient diagnosed with myelodysplastic syndrome. Peripheral blood samples were collected when the patient visited our hospital for his two out-patient follow-ups in May 2018. The 3-D tomograms of randomly chosen RBCs were reconstructed using a commercial ODT setup. From the reconstructed 3-D RBCs, 37.5% and 32.8% of RBCs demonstrated cup-like shapes at the first and the second out-patient follow-up, respectively. Even though this is a single case report, the finding is novel and can be a potential dyserythropoietic feature found in peripheral blood. KCI Citation Count: 0