In vitro antiprotozoal activity of organic and aqueous extracts of several Turkish Lamiaceae species
The in vitro antiprotozoal activities of methanolic extracts and subextracts prepared from the aerial parts of five Lamiaceae plants ( Salvia tomentosa Miller, S. sclarea L., S. dichroantha Stapf., Nepeta nuda L. subsp. nuda and Marrubium astracanicum Jacq. subsp. macrodon (Bornm.) P.H. Davis were e...
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Published in | Planta Medica |
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Main Authors | , , , , |
Format | Conference Proceeding |
Language | English |
Published |
05.08.2011
|
Online Access | Get full text |
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Summary: | The
in vitro
antiprotozoal activities of methanolic extracts and subextracts prepared from the aerial parts of five Lamiaceae plants (
Salvia tomentosa
Miller,
S. sclarea
L.,
S. dichroantha
Stapf.,
Nepeta nuda
L. subsp. nuda and
Marrubium astracanicum
Jacq. subsp.
macrodon
(Bornm.) P.H. Davis were evaluated against four parasitic protozoa,
Trypanosoma brucei rhodesiense, T. cruzi, Leishmania donovani
and
Plasmodium falciparum
. The cytotoxic potentials of the extracts on L6 cells were also evaluated. Melarsoprol, benznidazole, miltefosine, chloroquine and podophyllotoxin were used as reference drugs. MeOH extracts showed antiprotozoal potential against three or four parasites. Hence, they were dispersed in water and partitioned against n-hexane and chloroform, respectively, to yield three subextracts that were screened in the same test systems. The n-hexane extract of
N. nuda
was the most active against
T. brucei rhodesiense
with IC50 value of 0.62µg/mL, whilst the CHCl
3
extracts of
S. tomentosa
and
S. dichroantha
showed significant activity against
L. donovani
(IC
50
1.81 and 2.31µg/mL, respectively). All organic extracts displayed moderate trypanocidal potential against
T. cruzi
with hexane extract of
S. sclarea
being the most active one (IC50 18.17µg/mL). Again all organic extracts exhibited remarkable antimalarial activities and with IC50 values in the range of 2.54–3.78µg/ml, the chloroform subextracts appeared to be slightly more potent than the hexane subextracts (IC
50
values 3.37–4.64µg/mL). The extracts displayed low or no cytotoxicity towards mammalian L6 cells. This is the first study reporting the antimalarial, leishmanicidal and trypanocidal effects of the genera
Salvia, Nepeta
and
Marrubium
that are native to Turkey.
Keywords:
Lamiaceae, Nepeta, Salvia, Marrubium, Antiprotozoal activity |
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ISSN: | 0032-0943 1439-0221 |
DOI: | 10.1055/s-0031-1282865 |