Anti-inflammatory activity of Thai traditional medicine preparation called Prasaprohyai

• Published in: Planta Medica
• Main Authors: Itharat, A, Makchuchit, S, Tewtrakul, S
• Format: Conference Proceeding
• Language: English
• Published: 21.07.2009
• ISSN: 0032-0943; 1439-0221
• DOI: 10.1055/s-0029-1234860

Thai traditional medicine preparation called Prasaprohyai was commonly used to treat a cold, asthma and as antipyretic drug. It is composed with nineteen plants, Amomum testaceum , Anethum graveolens , Angelica dahurica , Angelica sinensis , Artemisia annua , Atractylodes lancea , Cuminum cyminum , Dracaena loureiri , Foeniculum vulgare , Kaempferia galanga , Lepidium sativum , Ligusticum sinense , Mammea siamensis , Mesua ferrea , Mimusops elengi , Myristica fragrans , Nelumbo nucifera , Nigella sativa and Syzygium aromaticum [1]. The objective of this research is to investigate on anti-inflammation activity of this preparation and its components. It and its components were extracted by ethanol, ethanol-water and water which imitated with using in Thai traditional book [1]. These extracts were examined for their inhibitory activities against lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW 264.7 cell lines. Nitric oxide in the culture supernatant was measured by Griess reaction [2]. The ethanolic extract of Prasaprohyai preparation showed high anti-inflammation with this assay (IC 50 =7.291µg/ml). The ethanolic extract of Myristica fragrans (Chan thet) which is an ingredient of this preparation exhibited the most potent inhibitory activity, with an IC 50 value of 1.613µg/ml, followed by the ethanolic extract of Ligusticum sinense (Kot hua bua) (IC 50 =3.769µg/ml) and Nigella sativa (Thian dam) (IC 50 =4.085µg/ml). The water and ethanol-water extracts of all plants were apparently inactive (IC 50 >100µg/ml). These results can support using Prasaprohyai in Thai traditional medicine for antipyretic caused by inflammation. References: [1] Foundation of resuscitate and encourage Thai Traditinal Medicine (2005) Thai Pharmaceutical Book Pikanate Printing Center Cooporation. [2] Tewtrakul, S. and Itharat, A. (2007)J. Ethnopharmacol. 109:412–416.