Development and validation of RP-HPLC method on Atovaqone and Proguanil in bulk and tablet dosage form

• Published in: Journal of Current Pharma Research Vol. 4; no. 1; pp. 1093 - 1096
• Main Authors: Patil, S D, Kokate, S D, Mohite, S K, Magdum, C S
• Format: Journal Article
• Language: English
• Published: Karad Human Journals 15.11.2013
• ISSN: 2230-7834; 2230-7842
• DOI: 10.33786/JCPR.2013.v04i01.008

A simultaneous RP-HPLC method is developed for the estimation of Atovaquone (ATV) and Proguanil (PRO) in tablet dosage form. Chromatography was carried on an Inertsil (ODS) 250×4.6mm, particle size 5µm, C18 column using isocratic composition of methanol as mobile phase A and 0.02M phosphate buffer as mobile phase B in ratio of 80:20 at a flow rate of 1ml/min with detection at 260 nm. The retention times of the ATV and PRO was about 10.62 and 3.83 min. respectively. The detector response is linear from 50-350µg/ml and 20-120µg/ml of test concentration for ATV and PRO respectively. The method was validated by determining its Linearity, accuracy and precision. The proposed method is simple, fast, sensitive, Linear, accurate, rugged and precise hence can be applied for routine quality control in bulk and in tablet dosage form. [PUBLICATION ABSTRACT]