RECOMBINATION OF TOMATO FUSARIUM WILT-RESISTANCE GENES

• Published in: HortScience Vol. 28; no. 5; p. 505
• Main Authors: Scott, J.W, Jones, John Paul, Brommonschenkel, S.H, Tanksley, S.D
• Format: Journal Article
• Language: English
• Published: 01.05.1993
• ISSN: 0018-5345; 2327-9834
• DOI: 10.21273/HORTSCI.28.5.505f

Tomato ( Lvcopersicon esculentum ) line E427 contains Fusarium wilt resistant gene I-3 on chromosome 7 and I-2 (and presumably the linked I ) genes on chromosome 11. E427 was crossed with `Bonny Best' ( i , i-2 , i-3 ) and backcrosses (BC) to `Bonny Best' and F 2 , seed were obtained. Self pollination of 187 BC and 150 F 2 plants were made. Progeny were screened against Fusarium races 1, 2, and 3 and lines with recombinant ratios were self-pollinated and rescreened until homozygous. Five lines were resistant to races 2 and 3 but susceptible to race 1. These had the isozyme band got-2 linked to I-3 , RFLP markers linked to I-3 and no RFLP markers linked to I-2 . Five lines were resistant to race 1 but susceptible to races 2 and 3. These had the susceptible qot-2 band and no RFLP markers linked to I-3 or I-2 . F 2 complementation tests of 2 of these lines with `Manapal' ( I ) indicated they contained I . Three lines were resistant to race 2 but susceptible to races 1 and 3. These had the susceptible qot-2 band, I-2 linked RFLP markers, and no I-3 linked RFLP markers.