HISTOCHEMICAL ASSESSMENT OF GROUND-BASED AND MIR-GROWN WHEAT

• Published in: HortScience Vol. 31; no. 6; p. 918
• Main Authors: Strickland, D.T, Biesinger, H, Campbell, W.F, Salisbury, F.B, Hole, P.S, Gillespie, L, Levinskikh, M, Ivanova, I
• Format: Journal Article
• Language: English
• Published: 01.10.1996
• ISSN: 0018-5345; 2327-9834
• DOI: 10.21273/HORTSCI.31.6.918E

Our objectives were to ascertain whether wheat ( Triticum aestivum L. cv. SuperDwarf) plants, grown aboard the Russian space station Mir in the microgravity of space, exhibited any plant structure and histochemical changes compared with those ground-based plants grown in Moscow, Russia, and Logan, Utah. Plants were harvested at stages of ontogeny corresponding to day 6, 14, 25, 35, and 55 post-emergence and placed in 4% formaldehyde: 1% glutaraldehyde (4F: 1G) fixative, adjusted to pH 7.2, and stored in Aclam plastic bags. Upon return to earth, samples were dehydrated and embedded in Spurr's resin. Use of differential chromophores on semi-thin sections (1 μm) suggests no major artifacts in cellular structure. Enzyme localizations for lignin, carbohydrate, starch, alkaline and acid phosphatase indicate that plants grown aboard Mir appeared to have less lignin than ground control plants. (Supported by NASA Grant NCC 2-831 and the Utah Agr. Expt. Station.)