GENETIC LINKAGES OF RAPD MARKERS IN SWEETPOTATO
Random amplified polymorphic DNA (RAPD) markers were analyzed in parents and progeny of four sweetpotato crosses. An average of 69 primers were tested and 23.5% produced well resolved polymorphic banding patterns. Each polymorphic primer had an average of 1.9 polymorphic bands resulting in 0.45 poly...
Saved in:
Published in | HortScience Vol. 29; no. 7; p. 727 |
---|---|
Main Authors | , |
Format | Journal Article |
Language | English |
Published |
01.07.1994
|
Online Access | Get full text |
Cover
Loading…
Summary: | Random amplified polymorphic DNA (RAPD) markers were analyzed in parents and progeny of four sweetpotato crosses. An average of 69 primers were tested and 23.5% produced well resolved polymorphic banding patterns. Each polymorphic primer had an average of 1.9 polymorphic bands resulting in 0.45 polymorphic fragments per primer tested. Phenotypic segregation ratios of 88% of polymorphic fragments fit those expected for hexaploid Mendelian inheritance. Numbers of linked polymorphic fragments and numbers of linkage groups were 13 and 5 for Cross A, 0 and 0 for Cross B, 23 and 3 for Cross C and 16 and 6 for Cross D. Those results indicated that RAPD markers have potential for a genetic linkage map in sweetpotato; however, many primers must be screened. |
---|---|
ISSN: | 0018-5345 2327-9834 |
DOI: | 10.21273/HORTSCI.29.7.727d |