IDENTIFICATION OF A RAPD MARKER LINKED TO THE ROOT KNOT NEMATODE-RESISTANT GENE IN SWEETPOTATO

The inheritance of root-knot nematode race 3 [ Meloidogyne incognita (Kofoid & White) Chitwood] resistance was studied in 71 progenies of the F 1 backcross population produced from the resistant parent `Regal' and the susceptible parent `Vardaman'. The distribution frequency of the pro...

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Bibliographic Details
Published inHortScience Vol. 31; no. 5; p. 747
Main Authors Ukoskit, Kittipat, Thompson, Paul G, Lawrence, Gary W, Watson, Clarence E
Format Journal Article
LanguageEnglish
Published 01.09.1996
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Summary:The inheritance of root-knot nematode race 3 [ Meloidogyne incognita (Kofoid & White) Chitwood] resistance was studied in 71 progenies of the F 1 backcross population produced from the resistant parent `Regal' and the susceptible parent `Vardaman'. The distribution frequency of the progenies measured on total nematode number (eggs + juveniles) indicated a bimodal distribution with a ratio of 4 resistant: 1 susceptible. Based on this phenotypic ratio, the proposed genetic model was duplex polysomic inheritance (RRrrrr = resistant). Bulk segregant analysis in conjunction with the RAPD technique was employed to identify RAPD marker linked to the root knot nematode-resistant gene. Nine of 760 random decamer primers screened showed polymorphic bands. Primer OPI5 1500 produced a band in the resistant bulk, but not in the susceptible bulk. Estimated recombination frequency of 0.24 between the OPI5 1500 marker and the root-knot nematode-resistant gene indicated linkage.
ISSN:0018-5345
2327-9834
DOI:10.21273/HORTSCI.31.5.747d