802 PB 106 PLANT REGENERATION THROUGH SOMATIC EMBRYOGENESIS AND ORGANOGENESIS IN GRAPE ROOT STOCK CULTIVAR AXR#1

• Published in: HortScience Vol. 29; no. 5; p. 548
• Main Authors: Waneck, Don, Mathews, H, Stamp, J, Bestwick, R
• Format: Journal Article
• Language: English
• Published: 01.05.1994
• ISSN: 0018-5345; 2327-9834
• DOI: 10.21273/HORTSCI.29.5.548b

Zygotic embryo explants of grape cultivar AXR#1 were isolated from maw-e seeds and cultured on medium supplemented with naphthoxy acetic acid beta-(NOA) and benzylaminopurine (BA). Embryo explants dedifferentiated to form embryogenic callus. Globular stage embryos were visible in 9-10 months. On transfer 10 a growth regulator free medium supplemented with charcoal these globular embryos underwent further stages of embryo development. In a period of 30-40 days embryogenic tissues turned into clumps of somatic embryos displaying different stages of development Cotyledonary stage embryos were separated and transferred to basal medium. These embryos developed into complete plants. Cold and desiccation treatment of somatic embryos significantly enhanced the rate of plant conversion. Hypocotyl segments of elongated somatic embryos were good source explant for induction of shoot organogenesis. The hypocotyl-length and the proximity to-shoot-apex were found to influence the rate of shoot induction from hypotyl segments. Multiple shoot complexes which formed on hypocotyl segments were separated and individual shoots were grown on a root induction medium resulting in complete plant development. The possibility of both embryogenic and organogenic modes of plant regeneration make somatic embryos a highly versatile explant source for experiments on genetic manipulation.