USE OF IN VITRO TECHNIQUES TO OVERCOME INCOMPATIBILITY IN SWEETPOTATO

To develop In vitro techniques to overcome incompatibility in sweetpotato the cross and self incompatible cultivars Regal and MD-708 were cross pollinated and also crossed with the compatible `Vardaman'. Observation of pollen behavior in different crosses after 3, 7, and 24 hours, showed good g...

Full description

Saved in:
Bibliographic Details
Published inHortScience Vol. 26; no. 5; p. 490
Main Authors Rojas, Salvador, Thompson, Paul G
Format Journal Article
LanguageEnglish
Published 01.05.1991
Online AccessGet full text

Cover

More Information
Summary:To develop In vitro techniques to overcome incompatibility in sweetpotato the cross and self incompatible cultivars Regal and MD-708 were cross pollinated and also crossed with the compatible `Vardaman'. Observation of pollen behavior in different crosses after 3, 7, and 24 hours, showed good germination and tube development in compatible crosses, but no germination in incompatibles. In a preliminary experiment using embryo rescue techniques plants were produced only from compatible crosses at 25 and 30 days after pollination. In subsequent experiments, immature embryos were rescued when cultured 15 days after pollination. The highest percentage of rescued embryos resulted from Murashige-Skoog medium. Intraovarian, stigmatic and placental in vitro fertilization were investigated to overcome incompatibility. Embryos were not formed from any of those methods, but callus was produced with placental pollination.
ISSN:0018-5345
2327-9834
DOI:10.21273/HORTSCI.26.5.490b